As you all know, the H1N1 virus (better known as the swine flu) was declared a pandemic in the human culture this year. Pandemics occur when an infectious disease spreads throughout the world to different continents. The swine flu itself is very interesting. It is a combination of three flu types: human, avian, and swine. The outbreak that occured recently was due in part to a reassortment of genes between strains from these organisms. While most individuals recover from symptoms, scientists are in the trial phase of preparing a vaccine that combines all of the strains from the flu. A vaccine is an agent that prepares the body for an immunological response. For example, to battle the flu it would be beneficial to include parts of the infectious causing agents (parts of the virus). Once the person has been exposed to a small amount of the virus, their immune system is prepared to battle the infection the second time it is encountered; making it easier for the immune system to recognize and kill the virus.
Now that you have a background, let's get to the real issue. Before a person can be diagnosed with the H1N1 virus, the virus must be detected. There are many ways to do this. An ELISA assay (which can test bodily fluid samples for antibodies to the virus or antigens from the virus) or a PCR assay would be ideal. PCR would amplify the DNA of the virus if it is present...which would ultimately provide information of how much of the virus is in the person's system. The latest technique being used today is Real-time PCR. RT PCR (or quantitative PCR) allows for quantification and detection of the DNA. The techniques takes PCR one step further; as the product accumulates, it is quantified...making it a quicker process. Usually quantification is done through fluorescent dye.
In the study listed below, they detect the H1N1 influenza A virus in controls and specimen samples. I did not go into great detail about PCR but I am listing what is needed for PCR (& RT PCR). This is exciting...with methods like RT PCR to help detect the virus, we can begin to
Here is the paper --> http://jcm.asm.org/cgi/reprint/JCM.01087-09v1?view=long&pmid=19553589
PCR requirements: primers (forward and reverse), DNA sample, thermocycler, Taq polymerase enzyme, Magnesium, Buffer, dNTPs
RT PCR requirements: everything from PCR and fluorescent dye
Tuesday, July 28, 2009
Sunday, July 26, 2009
ITWYLD
If today was your last day...
As I sat feeling depressed about my current situation, I thought listened to one of my favorite songs by Nickleback. It has an excellent point...not everyday is promised...yet individuals go about thinking as if it is...and here is why...
The society we live into today is ruled by one thing...MONEY. It's the root of all happiness and all evil. If you have money, you're "happy" because you know that you are capable of doing just about anything you please (legally). If you don't have any money, you feel depressed and all you can think about is how to get money or improve your situation.
I find myself in a position I did not think I would see in a while...and I'm tired of blaming the economy. This country's current economic situation is because of bad money management on the part of the previous presidents...not the current president. This country is based on capitalistic views...we must get money to suceed...by any means necessary...
I quote Malcolm X loosely because that was not the purpose of the statement. We are willing to kill, steal, etc. for a small piece of paper? Why? I blame this mostly on our fore "fathers" who enstilled in us that we can't have anything or get anywhere without the little piece of green paper. I HATE THIS IDEA! Why can't we be happy and satisfied with our lives as they are? We always want MORE...we always have to be the best at something...or rule everything...
I personally feel that we have too much competition and money problems in our society...this will surely bring down our country (as it is now)...
I'm depressed because of my current economic status...I want the finer things in life...but if I died today, would that even matter...
So, I'm taking a hard look at the woman in the mirror...and I'm asking her (for once) to become the better person she was always meant to be...to rise above it and look towards the cloud with the silver lining...
She wants to help those everywhere...and she knows that she will be able to soon enough =)
Nickleback --> If Today Was Your Last Day
http://www.youtube.com/watch?v=k-OlqERixVE
Michael Jackson --> Man in the Mirror
http://www.youtube.com/watch?v=SGeZYednWtI
As I sat feeling depressed about my current situation, I thought listened to one of my favorite songs by Nickleback. It has an excellent point...not everyday is promised...yet individuals go about thinking as if it is...and here is why...
The society we live into today is ruled by one thing...MONEY. It's the root of all happiness and all evil. If you have money, you're "happy" because you know that you are capable of doing just about anything you please (legally). If you don't have any money, you feel depressed and all you can think about is how to get money or improve your situation.
I find myself in a position I did not think I would see in a while...and I'm tired of blaming the economy. This country's current economic situation is because of bad money management on the part of the previous presidents...not the current president. This country is based on capitalistic views...we must get money to suceed...by any means necessary...
I quote Malcolm X loosely because that was not the purpose of the statement. We are willing to kill, steal, etc. for a small piece of paper? Why? I blame this mostly on our fore "fathers" who enstilled in us that we can't have anything or get anywhere without the little piece of green paper. I HATE THIS IDEA! Why can't we be happy and satisfied with our lives as they are? We always want MORE...we always have to be the best at something...or rule everything...
I personally feel that we have too much competition and money problems in our society...this will surely bring down our country (as it is now)...
I'm depressed because of my current economic status...I want the finer things in life...but if I died today, would that even matter...
So, I'm taking a hard look at the woman in the mirror...and I'm asking her (for once) to become the better person she was always meant to be...to rise above it and look towards the cloud with the silver lining...
She wants to help those everywhere...and she knows that she will be able to soon enough =)
Nickleback --> If Today Was Your Last Day
http://www.youtube.com/watch?v=k-OlqERixVE
Michael Jackson --> Man in the Mirror
http://www.youtube.com/watch?v=SGeZYednWtI
Saturday, July 25, 2009
Immunotherapy (IT) =D
Today's blog is about a project I did research on while in college. Immunotherapy.
Most of us know about the more common treatments for cancer. Chemotherapy and radiation are commonly used. Immunotherapy is common but not many people have heard about it. The issues with chemotherapy and radiation are numerous. For starters, chemo subjects all of the body's cells to a toxic regimen. Yes, the neoplastic (cancer) cells receives chemo to kill the cells but the other normal cells in the body receives chemo as well. This is why many cancer patients become ill from the treatment. Radiation is similar. Patients are subjected to high rays that kill other cells. So, what is left you say? Immunotherapy.
The treatment is simply remarkable. It's similar to training the body to recognize the cancer and kill the cancer off itself! Tumors have certain biomarkers (antigens) that leave them recognizable by physicians. I consider them great targets for immunotherapy. There are 2 kinds of this therapy: monoclonal antibody IT and radioactive antibody IT. Monoclonal antibody IT involves injecting antibodies with affinity to tumor markers (or antigens) into the patient. Once the anibodies attach, they have the ability to amount an immune response in which T cells and NK cells (immune cells that fight and battle infection in the body) can come along and kill these cells by endocytosis (engulfing), etc. Radioactive IT acts in a similar way in which antibodies conjugated with radioactive isotopes attach to tumor markers (antigens) and a dose of radioactive chemicals to the tumor. Both methods target the tumor directly and avoid affecting the other cells.
In my presentation, I talked about targeting MUC 1 antigen markers. While the idea was good (MUC 1 is a common tumor antigen), MUC 1 is found on other cells as well. So, my plan headed a little south there lol. But, those markers that are specific to certain cancers (ex. ETA in breast cancer) are great targets. Many of these therapies are being used today. Drugs such as Bevacizumab (IgG monoclonal antibody used in monoclonal IT) and Tositumomab (IgG antibody conjugated with the Iodine-131 radioactive isotope) have been deemed safe by the FDA.
So, you guys might be getting an information overload after all of that lol.
But something to remember is that there is always an alternative treatment. Sure, in most cases we might not have discovered it...but with any treatment in science (and anything in life) there is progress. And progress will help many individuals who suffer everyday. =)
Most of us know about the more common treatments for cancer. Chemotherapy and radiation are commonly used. Immunotherapy is common but not many people have heard about it. The issues with chemotherapy and radiation are numerous. For starters, chemo subjects all of the body's cells to a toxic regimen. Yes, the neoplastic (cancer) cells receives chemo to kill the cells but the other normal cells in the body receives chemo as well. This is why many cancer patients become ill from the treatment. Radiation is similar. Patients are subjected to high rays that kill other cells. So, what is left you say? Immunotherapy.
The treatment is simply remarkable. It's similar to training the body to recognize the cancer and kill the cancer off itself! Tumors have certain biomarkers (antigens) that leave them recognizable by physicians. I consider them great targets for immunotherapy. There are 2 kinds of this therapy: monoclonal antibody IT and radioactive antibody IT. Monoclonal antibody IT involves injecting antibodies with affinity to tumor markers (or antigens) into the patient. Once the anibodies attach, they have the ability to amount an immune response in which T cells and NK cells (immune cells that fight and battle infection in the body) can come along and kill these cells by endocytosis (engulfing), etc. Radioactive IT acts in a similar way in which antibodies conjugated with radioactive isotopes attach to tumor markers (antigens) and a dose of radioactive chemicals to the tumor. Both methods target the tumor directly and avoid affecting the other cells.
In my presentation, I talked about targeting MUC 1 antigen markers. While the idea was good (MUC 1 is a common tumor antigen), MUC 1 is found on other cells as well. So, my plan headed a little south there lol. But, those markers that are specific to certain cancers (ex. ETA in breast cancer) are great targets. Many of these therapies are being used today. Drugs such as Bevacizumab (IgG monoclonal antibody used in monoclonal IT) and Tositumomab (IgG antibody conjugated with the Iodine-131 radioactive isotope) have been deemed safe by the FDA.
So, you guys might be getting an information overload after all of that lol.
But something to remember is that there is always an alternative treatment. Sure, in most cases we might not have discovered it...but with any treatment in science (and anything in life) there is progress. And progress will help many individuals who suffer everyday. =)
Friday, July 24, 2009
Another CF?....Frustration
Tonight's blog is weird lol...
Some people have common names but my the combination of my first and last names are not that common. So, to find someone with the exact same first name and almost the same last name is crazy! I mean there are variations of my last name but for some other girl to have the first name too...simply bizarre...
Now on to other important matters...
I must say that my life for the past 3 months has not been the best...Yes I am a college graduate...but I am a graduate who is still out of work.
I have more than enough passion and intelligence to offer any laboratory; and yet I find myself working in a position that I do not like. I want to be happy but I simply can not. How can ANYONE truely understand unless they experience the everyday struggle that I go through at home and at work...
I try to be happy for my friends...but I can't help but ask why those same good fortunes that are blessing them are not blessing me...what have I done wrong? I have faith and I believe in myself...I'm passionate...I'm an upstanding citizen...I have a degree in a field that many want to attain but find it difficult to...I'm in a field that favors me in every aspect...and I graduated from a GREAT institute...so why is this happening to me?
I believe in God...I have faith...I have dreams...why?
I know it could be worse...but for me this is worse...I'm a person who thrives off success...who lives for the ability to conduct research in a lab...to learn...to improve...to be better....
and yet I see myself down every day...
YES, I am happy for those who make it...but I cringe a little inside because I have not...I AM THE HARDEST WORKING PERSON I KNOW...
I don't knock others, but I know myself. I am the type of person that if it takes working a job at 30,000 a year in order to get experience to earn a degree that would get me 90,000 a year, I'm willing to do that...
Even at the job I'm at now I work had...
So now I find myself at a crossroads...the only thing that will ease my pain is prayer and the occasional laugh from Family Guy or Spongebob cartoons...
To quote Drake loosely...I know the success is coming, I just hope that I'm alive for it... =?
DrCBri OUT
Thursday, July 23, 2009
Stem Cell Research!
http://www.time.com/time/health/article/0,8599,1912375,00.html
The above article was published in TIME. It talks about a research group producing mice from stem cells that have not been talked about too much.
Stem cells are cells that have the ability to differentiate into specialized cells (for instance erythrocytes/red blood cells) and also have the ability to renew themselves through mitotic division. Most of the stem cell talk in the news has been about embryonic stem cells. These source for these cells is the blastocyst (or early embryo). These cells come at the risk of the embryo itself...which is why the issue is so controversial. These cells are totipotent and have the ability to become an individual.
The cells used in this experiment however are adult stem cells which were induced or reprogrammed to grow into mice. This is great because adult stem cells are multipotent; while they can give rise to other cells, they are limited to what type of cells they can give rise to. So for these cells to be able to give rise to an organism is fascinating. It must be stressed that I do not condone the "cloning" aspect of this experiment. However I do condone the significance of being able to treat many of the diseases that humans suffer (ex. Parkinson's disease). Read the article to get more info =)
The above article was published in TIME. It talks about a research group producing mice from stem cells that have not been talked about too much.
Stem cells are cells that have the ability to differentiate into specialized cells (for instance erythrocytes/red blood cells) and also have the ability to renew themselves through mitotic division. Most of the stem cell talk in the news has been about embryonic stem cells. These source for these cells is the blastocyst (or early embryo). These cells come at the risk of the embryo itself...which is why the issue is so controversial. These cells are totipotent and have the ability to become an individual.
The cells used in this experiment however are adult stem cells which were induced or reprogrammed to grow into mice. This is great because adult stem cells are multipotent; while they can give rise to other cells, they are limited to what type of cells they can give rise to. So for these cells to be able to give rise to an organism is fascinating. It must be stressed that I do not condone the "cloning" aspect of this experiment. However I do condone the significance of being able to treat many of the diseases that humans suffer (ex. Parkinson's disease). Read the article to get more info =)
Wednesday, July 22, 2009
Cancer Research =)
I'm reposting this because I feel like it should stand on its own
On a brighter note, I read an interesting article on CNN about triple negative breast cancer(TNBC). I want to go into cancer research later in life. I plan on obtaining my doctorate in Cancer Bio...which would explain why I'm suggesting the article lol. TNBC is a type of breast cancer that is non responsive to hormone therapy because the tumor lacks estrogen, progesterone, and HER2 hormone receptors. The cancer is very difficult to treat...although traditional forms of treatment such as chemotherpy and radiation are used. The cancer is very aggressive and seen in young African American females. The doctor in the article travels to Africa (specifically Ghana) to see if she can find a clue or some significance (connection) with the cancer seen in women in Ghana and in the US. Even though this is a small step, this is big. Not only may there be genetic connections, but this could be a step towards finding a more effective diagnosis in the future. There are so many new techniques being used to diagnose various cancer (ex. ovarian)...I studied Mass Spec in my Biochem class and that is helping to diagnose patients in enough time to receive treatment before they succumb to the disease. Cancer is a crazy disease...it can be virtually untreatable at times and is uncurable...It's up to the new scientists coming up to stop this...and let me say that I am ready for the challenge =)
http://www.cnn.com/2009/HEALTH/07/21/bia.triple.negative.cancer/index.html
On a brighter note, I read an interesting article on CNN about triple negative breast cancer(TNBC). I want to go into cancer research later in life. I plan on obtaining my doctorate in Cancer Bio...which would explain why I'm suggesting the article lol. TNBC is a type of breast cancer that is non responsive to hormone therapy because the tumor lacks estrogen, progesterone, and HER2 hormone receptors. The cancer is very difficult to treat...although traditional forms of treatment such as chemotherpy and radiation are used. The cancer is very aggressive and seen in young African American females. The doctor in the article travels to Africa (specifically Ghana) to see if she can find a clue or some significance (connection) with the cancer seen in women in Ghana and in the US. Even though this is a small step, this is big. Not only may there be genetic connections, but this could be a step towards finding a more effective diagnosis in the future. There are so many new techniques being used to diagnose various cancer (ex. ovarian)...I studied Mass Spec in my Biochem class and that is helping to diagnose patients in enough time to receive treatment before they succumb to the disease. Cancer is a crazy disease...it can be virtually untreatable at times and is uncurable...It's up to the new scientists coming up to stop this...and let me say that I am ready for the challenge =)
http://www.cnn.com/2009/HEALTH/07/21/bia.triple.negative.cancer/index.html
Eyecare Eye wear =)
http://www.cnn.com/2009/TECH/science/07/22/contact.lens.drugs/index.html
The above article is something I came across today. Brief intro...
Most medication for eyes are dispensed through eye drops. Most individuals (including myself) have difficulties delivering eye drops to the eye. The anticipation of actually putting th drop in the eye and making sure that the drop goes into your eye rather than down your cheek is frustrating. Many times most of the medication fails to reach the eye.
In comes Science! =)
Scientists are now developing a contact lenses that includes the medication needed for certain eye conditions. Individuals with such eye diseases as glaucoma would simply be able to place the lense in their eye and medication is steadily released! Wow!
I'm slightly interested in opthamalogy/optometry so this is great!
Thank you science once again =)
The above article is something I came across today. Brief intro...
Most medication for eyes are dispensed through eye drops. Most individuals (including myself) have difficulties delivering eye drops to the eye. The anticipation of actually putting th drop in the eye and making sure that the drop goes into your eye rather than down your cheek is frustrating. Many times most of the medication fails to reach the eye.
In comes Science! =)
Scientists are now developing a contact lenses that includes the medication needed for certain eye conditions. Individuals with such eye diseases as glaucoma would simply be able to place the lense in their eye and medication is steadily released! Wow!
I'm slightly interested in opthamalogy/optometry so this is great!
Thank you science once again =)
Tuesday, July 21, 2009
*SIGH*
I wish that this blog would be better but it can't. Today has been a long stressful day. To begin with, my cousin betrayed a discrete agreement. She asked me to do something...and went behind my back and told about the agreement. I was utterly upset. Then, she asked me to help her today...I did but relunctantly. Why should I help you now that the agreement is pretty much null and void. Now that everything is done, I can be honest. I didn't want to help at all! But out of the goodness of my heart I did.
If that wasn't enough, my uncle calls my mom and upsets her about a comment she SUPPOSEDLY made to my great uncle. My mom simply called my uncle sending condolences about his dead wife...now I'm upset and my anxiety is running wild...
I feel like my life is in shambles...I want to move away...I want to live in laboratory doing research until my eyes bleed...I'm tired of the drama...it never fails...and as my situation gets worse, the only thing that I can do is calm myself and hope and pray that things will get better...
On a brighter note, I read an interesting article on CNN about triple negative breast cancer(TNBC). I want to go into cancer research later in life. I plan on obtaining my doctorate in Cancer Bio...which would explain why I'm suggesting the article lol. TNBC is a type of breast cancer that is non responsive to hormone therapy because the tumor lacks estrogen, progesterone, and HER2 hormone receptors. The cancer is very difficult to treat...although traditional forms of treatment such as chemotherpy and radiation are used. The cancer is very aggressive and seen in young African American females. The doctor in the article travels to Africa (specifically Ghana) to see if she can find a clue or some significance (connection) with the cancer seen in women in Ghana and in the US. Even though this is a small step, this is big. Not only may there be genetic connections, but this could be a step towards finding a more effective diagnosis in the future. There are so many new techniques being used to diagnose various cancer (ex. ovarian)...I studied Mass Spec in my Biochem class and that is helping to diagnose patients in enough time to receive treatment before they succumb to the disease. Cancer is a crazy disease...it can be virtually untreatable at times and is uncurable...
It's up to the new scientists coming up to stop this...and let me say that I am ready for the challenge =)
http://www.cnn.com/2009/HEALTH/07/21/bia.triple.negative.cancer/index.html
I wish that this blog would be better but it can't. Today has been a long stressful day. To begin with, my cousin betrayed a discrete agreement. She asked me to do something...and went behind my back and told about the agreement. I was utterly upset. Then, she asked me to help her today...I did but relunctantly. Why should I help you now that the agreement is pretty much null and void. Now that everything is done, I can be honest. I didn't want to help at all! But out of the goodness of my heart I did.
If that wasn't enough, my uncle calls my mom and upsets her about a comment she SUPPOSEDLY made to my great uncle. My mom simply called my uncle sending condolences about his dead wife...now I'm upset and my anxiety is running wild...
I feel like my life is in shambles...I want to move away...I want to live in laboratory doing research until my eyes bleed...I'm tired of the drama...it never fails...and as my situation gets worse, the only thing that I can do is calm myself and hope and pray that things will get better...
On a brighter note, I read an interesting article on CNN about triple negative breast cancer(TNBC). I want to go into cancer research later in life. I plan on obtaining my doctorate in Cancer Bio...which would explain why I'm suggesting the article lol. TNBC is a type of breast cancer that is non responsive to hormone therapy because the tumor lacks estrogen, progesterone, and HER2 hormone receptors. The cancer is very difficult to treat...although traditional forms of treatment such as chemotherpy and radiation are used. The cancer is very aggressive and seen in young African American females. The doctor in the article travels to Africa (specifically Ghana) to see if she can find a clue or some significance (connection) with the cancer seen in women in Ghana and in the US. Even though this is a small step, this is big. Not only may there be genetic connections, but this could be a step towards finding a more effective diagnosis in the future. There are so many new techniques being used to diagnose various cancer (ex. ovarian)...I studied Mass Spec in my Biochem class and that is helping to diagnose patients in enough time to receive treatment before they succumb to the disease. Cancer is a crazy disease...it can be virtually untreatable at times and is uncurable...
It's up to the new scientists coming up to stop this...and let me say that I am ready for the challenge =)
http://www.cnn.com/2009/HEALTH/07/21/bia.triple.negative.cancer/index.html
Sunday, July 19, 2009
Ok...I don't get it
I'm usually not the type of person to post 2 times in one day but something disturbing occurred recently. My friend called me to tell me about how her life was going. I love my friend...but I realized something...SHE DOESN'T HAVE THE SAME DRIVE AS ME.
Right now, I'm job hunting. And although things are bleak, I have an undieing passion within me. I want to be successful. It's not just about the finer things in life either...it's about what God put me on the Earth to do. And I now know what that is...and I am passionate about it and about acheiving that goal. I'm the type of person that if I have to work harder than 1 million people to get what I want, I will. Because I know that things will work out if I do what I need to do. I recently took a short term job to help to pay finances in the household. And while my friend had the same opportunity to do this, she didn't. I was hurt, upset, and dissapointed. I had defended this person for so long...saying that no she wasn't lazy, she was just delayed due to harsh life situations. Now I realize that I can't help someone I care for if they do not want to be helped. She could have helped her situation more...but she didn't. In fact she's gotten herself into more trouble and I honestly don't think she'll get out as soon as she'd like...
Sure I want to have it all like everyone else...but nothing comes withought hard work and dedication. I am an intelligent woman...and while there are others that may be smarter than me, I know that they'll never be able to out work me. The moral of this post is to push through everything and anything...don't be lazy, be on top of your game...if you want to be successful, you gotta be diligent and patient
Drake --> Successful
http://www.youtube.com/watch?v=7kcDpsjbCok
Right now, I'm job hunting. And although things are bleak, I have an undieing passion within me. I want to be successful. It's not just about the finer things in life either...it's about what God put me on the Earth to do. And I now know what that is...and I am passionate about it and about acheiving that goal. I'm the type of person that if I have to work harder than 1 million people to get what I want, I will. Because I know that things will work out if I do what I need to do. I recently took a short term job to help to pay finances in the household. And while my friend had the same opportunity to do this, she didn't. I was hurt, upset, and dissapointed. I had defended this person for so long...saying that no she wasn't lazy, she was just delayed due to harsh life situations. Now I realize that I can't help someone I care for if they do not want to be helped. She could have helped her situation more...but she didn't. In fact she's gotten herself into more trouble and I honestly don't think she'll get out as soon as she'd like...
Sure I want to have it all like everyone else...but nothing comes withought hard work and dedication. I am an intelligent woman...and while there are others that may be smarter than me, I know that they'll never be able to out work me. The moral of this post is to push through everything and anything...don't be lazy, be on top of your game...if you want to be successful, you gotta be diligent and patient
Drake --> Successful
http://www.youtube.com/watch?v=7kcDpsjbCok
Life I wonder...will it take me under?
So...hello audience lol
Well, here's my "formal" intro. I'm a girl who's been through life's challenges and now I find myself in a position in which I would rather talk about them over the internet than to people. I find that in general, PEOPLE SUCK...
I think everyone comes to this realization after the initial embarrassment that is experienced as a child when kids stare at you because you different. I must say that after 16 years of education, this conclusion does not change once you get older. In fact it gets worst. Think about it, being a part of a country who's moto is "if you're not with us, you're against us" has definitely put a perspective on things.
So now, I come to the point in my life where I know what I want to do and yet I'm denied it every step of the way...so let me set the record straight...no I'm not saying that I am above reproach for my life and my mistakes...but I am saying that I did what needed to be done and I'm in a position that I never thought I'd be in...
To you out there who feel the same, this blog is for you..because of course it could be so much worst than this...lol
Well, here's my "formal" intro. I'm a girl who's been through life's challenges and now I find myself in a position in which I would rather talk about them over the internet than to people. I find that in general, PEOPLE SUCK...
I think everyone comes to this realization after the initial embarrassment that is experienced as a child when kids stare at you because you different. I must say that after 16 years of education, this conclusion does not change once you get older. In fact it gets worst. Think about it, being a part of a country who's moto is "if you're not with us, you're against us" has definitely put a perspective on things.
So now, I come to the point in my life where I know what I want to do and yet I'm denied it every step of the way...so let me set the record straight...no I'm not saying that I am above reproach for my life and my mistakes...but I am saying that I did what needed to be done and I'm in a position that I never thought I'd be in...
To you out there who feel the same, this blog is for you..because of course it could be so much worst than this...lol
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